A new class of antimicrobial therapeutics targeting the envelope stress response of Gram-negative bacteria: abridged secondary publication
SW Tang1, SH Kwok1, X Li1, KH Tang1, JA Kubi2, AS Brah2, K Yeung2, M Dong1, YW Lam3
1 Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China
2 Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong SAR, China
3 School of Applied Science, University of Huddersfield, United Kingdom
  1. BING is a novel amphipathic peptide with broad-spectrum antibacterial activity. Genome-wide transcriptomic analysis suggests that BING represents a new class of antibiotics.
  2. BING suppresses the expression of genes involved in flagellar biosynthesis and chemotaxis, thereby inhibiting motility, in Escherichia coli and Salmonella enterica serovar Typhimurium.
  3. BING has synergistic effects with ampicillin, amoxicillin, and novobiocin in E. coli and Pseudomonas aeruginosa; it can suppress ampicillin resistance in P. aeruginosa.
  4. Amidation and D-amino acid substitution can stabilise BING against serum degradation and heat inactivation.
  5. BING and its derivatives are non-haemolytic and exhibit low in vivo toxicity.