Antioxidative effect of Gastrodiae Rhizoma–containing herbal formula in PC12 cell model: abridged secondary publication
Z Liu, CH Ko, CF Ng, HL Wong, JF Zhang, PK Lam, WS Poon, PC Leung
1Institute of Chinese Medicine, The Chinese University of Hong Kong
2State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong
3Department of Orthopaedic and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital
4Department of Surgery, The Chinese University of Hong Kong, Prince of Wales, Hospital
1. The IC50 of hydrogen peroxide (H2O2) was 61.9 μM
on PC12 cells.
2. Cell viability after co-treatment with Gastrodiae Rhizoma–containing herbal formula (DCXF) and H2O2 at all tested concentrations was similar to that with H2O2 treatment alone, suggesting that the antioxidative effect of DCXF is weak.
3. The antioxidant potential of DCXF was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. DCXF dose-dependently inhibited the DPPH radical at concentrations of 0.3125 to 20 mg/mL, compared with the control (0 mg/mL DCXF). However, the DPPH radical scavenging activity of DCXF was three orders of magnitude weaker than vitamin C.
2. Cell viability after co-treatment with Gastrodiae Rhizoma–containing herbal formula (DCXF) and H2O2 at all tested concentrations was similar to that with H2O2 treatment alone, suggesting that the antioxidative effect of DCXF is weak.
3. The antioxidant potential of DCXF was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. DCXF dose-dependently inhibited the DPPH radical at concentrations of 0.3125 to 20 mg/mL, compared with the control (0 mg/mL DCXF). However, the DPPH radical scavenging activity of DCXF was three orders of magnitude weaker than vitamin C.