A new class of antimicrobial therapeutics
targeting the envelope stress response of Gram-negative bacteria: abridged secondary publication
SW Tang1, SH Kwok1, X Li1, KH Tang1, JA Kubi2, AS Brah2, K Yeung2, M Dong1, YW Lam3
1 Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China
2 Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong SAR, China
3 School of Applied Science, University of Huddersfield, United Kingdom
- BING is a novel amphipathic peptide with broad-spectrum antibacterial activity. Genome-wide transcriptomic analysis suggests that BING represents a new class of antibiotics.
- BING suppresses the expression of genes involved in flagellar biosynthesis and chemotaxis, thereby inhibiting motility, in Escherichia coli and Salmonella enterica serovar Typhimurium.
- BING has synergistic effects with ampicillin, amoxicillin, and novobiocin in E. coli and Pseudomonas aeruginosa; it can suppress ampicillin resistance in P. aeruginosa.
- Amidation and D-amino acid substitution can stabilise BING against serum degradation and heat inactivation.
- BING and its derivatives are non-haemolytic and exhibit low in vivo toxicity.