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Rearrangement of the myeloid-lymphoid leukaemia
gene in Hong Kong Chinese children with acute leukaemia
BMW Lit, KF Wong, YL Kwong
Department of Pathology, Queen Elizabeth Hospital, 30 Gascoigne
Road, Kowloon, Hong Kong
OBJECTIVE. To determine the frequency of rearrangement
of the myeloid-lymphoid leukaemia gene in acute leukaemia in Hong
Kong Chinese children.
DESIGN. Immunophenotyping, cytogenetic, and molecular analysis.
SETTING. Regional hospital, Hong Kong.
PARTICIPANTS. Bone marrow or peripheral blood samples were taken
from 27 children aged 16 years or younger with acute leukaemia,
from September 1995 through February 1998.
MAIN OUTCOME MEASURES. Gene rearrangement was analysed by Southern
blotting of HindIII digestion products of mononuclear cell DNA,
followed by hybridisation with the myeloid-lymphoid leukaemia P/S4
probe. Nested reverse transcription-polymerase chain reaction analysis
was performed to detect and characterise duplication of the myeloid-lymphoid
leukaemia gene.
RESULTS. Only one (4%) of 23 children whose marrow or peripheral
blood samples contained adequate material for genetic study showed
rearrangement in the myeloid-lymphoid leukaemia gene. No children
were positive for partial tandem duplication of the myeloid-lymphoid
leukaemia gene.
CONCLUSION. Myeloid-lymphoid leukaemia gene rearrangement is rare
in Hong Kong Chinese children with acute leukaemia.
Hong Kong Med J 2001;7:9-14
Key words: Child; Chromosomes; Gene rearrangement;
Hong Kong; Leukemia, lymphocytic, acute/genetics; Leukemia, myeloid/genetics
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