Hong Kong Med J 2015;21(Suppl 4):S8-11
Direct identification and quantification of host and viral miRNAs after influenza infection using the next generation ultra-high throughput DNA sequencer
MY Lee, S Lok, CY Cheung
Centre of Influenza Research, Li Ka Shing Faculty of Medicine, The University of Hong Kong
1. The host and viral whole transcriptomes (including miRNA and mRNA of pathogenic H5N1 and seasonal H1N1 influenza-virus-infected human macrophages) were quantified and compared.
2. A total of 43 new human miRNA candidates were discovered.
3. No miRNA was encoded by influenza viruses.
4. In response to H5N1 infection, the RIG-I-like receptor signalling pathway was the most significant pathway regulated by known human miRNAs.