Hong Kong Med J 2015;21(Suppl 4):S31-4
Viral mutant discovery in hepatitis B virus quasi-species in patients undergoing long-term lamivudine treatment
CM Ding, JJY Sung, HLY Chan, J Luan
Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong
1. Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is an accurate method to detect drug-resistant mutations and identify novel mutations in chronic hepatitis B patients treated with lamivudine.
2. MALDI-TOF MS can simultaneously detect multiple mutations in a target sequence and at proportions as low as 10%.