Hong Kong Med J 2014 Aug;20(4):313–6 | Epub 6 Jun 2014
DOI: 10.12809/hkmj134190
REVIEW ARTICLE
An update on irreversible electroporation of liver tumours
Enoch SL Yeung, BDS, MB, BS; Max WY Chung, MB, BS; Keedon Wong, MB, BS; Clement YK Wong, MB, BS; Enoch CT So, BEng (UNSW), MB, BS; Albert CY Chan, FCSHK, FHKAM (Surgery)
Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong
 
Corresponding author: Dr Albert CY Chan (acchan@hku.hk)
 Full paper in PDF
Abstract
Objective: To investigate the clinical efficacy and safety of irreversible electroporation for ablation of liver tumour in humans.
 
Data sources: The PubMed and MEDLINE databases were systematically searched.
 
Study selection: Clinical research published in English in the last 10 years until October 2013 that address clinical issues related to irreversible electroporation of human liver tumours were selected. “Liver tumour”, “local ablative therapy”, and “irreversible electroporation” were used as the search terms.
 
Data extraction and synthesis: The data extracted for this review was analysed by the authors, with a focus on the clinical efficacy and the safety of irreversible electroporation. The complete response rates look promising, ranging from 72% to 100%, except in one study in a subgroup of liver tumours in which the complete response rate was only 50% that was likely due to the inclusion of larger-size tumours. In one study, the local recurrence rate at 12 months was approximately 40%. As for the safety of irreversible electroporation, there were only a few reported complications (cardiac arrhythmia, pneumothorax, and electrolyte disturbance) that were mostly transient and not serious. There was no reported mortality related to the use of irreversible electroporation.
 
Conclusion: Irreversible electroporation is a potentially effective liver tumour ablative therapy that gives rise to only mild and transient side-effects. Further studies with better patient selection criteria and longer follow-up are needed to clarify its role as a first-line liver tumour treatment modality.
 
 
Introduction
Local ablative therapies are frequently employed for the treatment of primary and secondary malignancies in the liver. Common choices include radiofrequency and microwave ablation. These treatment modalities, however, may cause thermal injury to major bile ducts within periductal tumours. In addition, the efficacy of thermal ablative therapy is often undermined by the heat sink effect, whereby the delivered thermal energy is dissipated via a continuous high blood flow in nearby major portal pedicles and hepatic veins. Recently, the use of irreversible electroporation (IRE) has been introduced into clinical practice. The aim of this article was to provide an updated review of the latest developments of this new technology in the management of liver tumours.
 
Methods
A search on the medical literature was performed to identify the relevant studies and reviews regarding the use of IRE as a treatment for primary liver neoplasm. Both PubMed and MEDLINE databases were searched for clinical studies published in English in the last 10 years (until October 2013) that involved the use of IRE for liver tumours. Key words used for the literature search were: “liver tumour”, “local ablative therapy”, and “irreversible electroporation”.
 
Mechanism and history of electroporation
Electroporation utilises electrical fields to induce changes to plasma membrane permeability. More specifically, multiple rapid direct current electrical pulses are applied to the tissue of interest. These electrical pulses induce nano-scale pores within the phospholipid bilayer, thus changing cell permeability.1 There are two types: reversible electroporation (RE) and IRE.
 
As the name suggests, tissues subjected to RE remain viable after the procedure. The lesser electrical strength and duration of the applied pulses during the procedure allow pores in the membrane to spontaneously seal by themselves. Researchers exploited this unique effect of a transient increase in cell permeability to enable foreign materials that were previously deemed impermeable to pass through the phospholipid bilayer. A prominent example would be electrochemotherapy. Since the 1990s, multiple human clinical trials2 3 4 have shown that RE applied for this purpose enhances the delivery of chemotherapy (eg bleomycin) to the desired tissue (eg skin cancer, breast cancer).
 
By contrast, IRE relies on delivering electrical pulses whose strength and/or duration exceeds the threshold of spontaneous cell membrane repair. The permanent permeability of the cell membrane that they induce disrupts the homeostasis of the cells, leading to cell death. Interestingly, this technique was largely ignored by the medical community until 2005, when Davalos et al5 proved its theoretical basis via a mathematical analysis. This predicted that “irreversible electroporation can ablate substantial volumes of tissue, comparable to those achieved with other ablation techniques, without causing any detrimental thermal effects and without the need of adjuvant drugs.”5
 
Rubinsky et al6 performed the first experimental IRE in 2007 by performing 35 ablations on 14 swine livers. Interestingly, all animals survived till the electrical pulse applications ceased, but reversible chemical paralysis was necessary to prevent unwanted muscular contractions during the procedure. Histologically there was haemorrhagic necrosis of the liver, but preservation of the vessels and bile ducts within the zones of ablation.7
 
Since then, many other animal studies have been performed in various organs and tissues, including liver, prostate, pancreas, small bowel, kidney, carotid artery, atrial appendages, and lung. The results were encouraging, in that IRE conferred three key advantages in all of these studies:
(1) It was effective in ablating tissues of interest, including tumours.
(2) It lacked the heat-sink effect. Traditional thermal ablation relied on tissue temperature reaching a certain threshold (60°C) in order to induce cell death. Cells near the large vessels were therefore prone to the continuously cooling effect from the flowing fluids within the vessels. This leads to incomplete necrosis, making local recurrence of tumours more likely.1
(3) It was capable of preserving critical structures (blood vessels, bile ducts, urethra, and nerves) within the zone of ablation. Maor et al8 performed an experiment by applying IRE directly onto the carotid arteries of six rats. All rats survived with no apparent side-effects. Carotid arteries remained intact with no evidence of aneurysm, thrombus, or necrosis 28 days later. Histologically, there was a statistically significant decrease in mean vascular smooth muscle cell density (24 ± 11 vs 139 ± 14; P<0.001) but with no apparent damage to extracellular matrix components and structure. This may explain why critical structures appear to be preserved with this new technology.
 
Clinical efficacy of irreversible electroporation on human liver tumours
To date, four published case series9 10 11 12 have evaluated the safety and efficacy of IRE on human liver tumours. All of them adopted the NanoKnife system (AngioDynamics, New York, US), which consists of a footswitch, a control panel with a screen and a cardiac synchroniser, and a direct current generator connected with unipolar or bipolar needle electrodes. The number and placement of electrodes are determined by the size of the target tumour. The current, the applied voltage, and duration of ablation can be varied according to tumour characteristics. The procedure can be performed via percutaneous, laparoscopic, or open surgical approaches.9 10 11
 
The Table9 10 11 12 illustrates the efficacy and postoperative outcomes after IRE in various case series. The complete response rate ranged from 72% to 100%,9 10 11 12 except in Thomson et al’s study9 in which the complete response rate was only 50% for colorectal liver metastasis. That study also demonstrated lack of significant tumour response when the size of the liver metastatic lesion was larger than 5 cm.
 

Table. Clinical efficacy of irreversible electroporation for liver tumours
 
In Cannon et al’s study,10 however, the complete response rates were the same for hepatocellular carcinoma (HCC) and colorectal liver metastasis. The lower complete response rate in Thomson et al’s study9 could be partially attributed to the inclusion of larger-size tumours and greater use of the percutaneous approach. Consistent with Thomson et al’s study,9 they also showed a trend towards higher recurrence rates for tumours exceeding 4 cm in diameter.
 
The longest follow-up among these studies was 12 months, which was in Cannon et al’s study,10 by which time the local recurrence rate was about 40% for both HCC and colorectal liver metastases. To date, no randomised controlled trial comparing the efficacy of IRE and other ablation modalities has been published. The latest quoted figures for recurrence rates are approximately 2% to 15% 2 years after radiofrequency ablation, and 11% to 35% 2 years after percutaneous ethanol injection.13
 
The complete response rate of liver tumour to IRE looked promising, but a 40% local recurrence rate after 1 year was too high to justify its use as first-line treatment. Moreover, tumour size seems to be an important consideration affecting the outcome. The inclusion of large tumours may have contributed to the high local recurrence rate in Cannon et al’s study.10 More prospective studies are warranted to define standard selection criteria in order to achieve satisfactory outcomes.
 
Adverse effects of irreversible electroporation
Like any other local ablative therapy, IRE is also associated with a few other adverse effects, be they general or procedure-related.
 
General intra-operative complications
As with all operations, IRE carries the risk of general anaesthesia9 and positional neuropraxia.14 In two retrospective studies, such effects occurred in an isolated number of patients but were transient and self-limiting, and resolved without any long-term disability.9 14
 
Specific intra-operative complications
One of the specific complications related to IRE is unintended injury to other organs and structures during manipulation of the electrodes. One instance of pneumothorax due to direct injury caused by an electrode was reported by Thomson et al.9 That pneumothorax resolved spontaneously and did not result in a delayed discharge. The same authors9 reported another instance of direct damage by an instrument due to an unplanned tip insertion during an attempt to treat a renal tumour. That particular patient had transient acute hypotension, and subsequent mild hypotension for a further 2 months.
 
Cardiac arrhythmia is a potential life-threatening complication associated with IRE, which is presumed due to application of a large current close to the heart, especially for liver tumours situated below the right hemidiaphragm. Thomson et al9 and Ball et al14 reported cases of ventricular bigeminy, ventricular tachycardia, and atrial fibrillation during the procedure, in which a drop in blood pressure was associated with the arrhythmia. Furthermore, IRE has been incorporated with electrocardiographic synchronisation, rendering the possibility of intra-operative arrhythmia less common. Although none of the studies reported mortality due to cardiac arrhythmia, this potentially devastating effect should not be ignored.
 
In addition to cardiac arrhythmia, discharges from the electrode could cause muscle stimulation. In one case, insufficient muscle relaxant was used, resulting in an upper body contraction similar to what ensues during a grand mal seizure.14 Hence, IRE treatment should be performed under general anaesthesia with deep neuromuscular blockade, in order to prevent excessive body movement during treatment.
 
Since IRE involves the disruption of the cellular membrane, it results in the release of intracellular contents whenever tumour cells are electroporated. Ball et al14 reported four instances of hyperkalaemia in 21 patients treated with IRE, but without significant sequelae. Early postoperative arterial blood gas sampling and electrocardiographic monitoring during the procedure may help to prevent the lethal consequence of severe hyperkalaemia.
 
Premature termination of the procedure for technical reasons has also been reported, but detailed explanations were not given.11 This complication subjects patients to further IRE treatments, but under more controlled conditions.
 
Postoperative complications
The postoperative complications of IRE have been reported in several studies. Postoperative pain was of primary concern for most of the operations. About half of the patients who underwent IRE had some degree of postoperative pain but those treated with either IRE or radiofrequency ablation reported similar pain scores.15 Notably, there have been no reported instance of vascular or biliary complications after IRE for periductal tumours or tumours abutting major vessels. This means that for tumours in difficult locations, it is a promising local ablative treatment modality compared with other local ablative therapies. Besides, hitherto there has not been any mortality directly related to IRE. One study reported that the mortality rate at 30 days was 0%.9 However, another study available in abstract form reported one fatality 1 month after the operation, though no other details were provided.16 As of 2012, IRE has been performed 158 times in 106 patients with liver tumours, with no attributed mortality.7 At the time of writing, a prospective multicentre phase II study on the efficacy and safety profile of the NanoKnife System (AngioDynamics) for early-stage HCC has just been completed and the outcome of this study is eagerly awaited.
 
Conclusion
The tumour ablative effect of IRE appears promising. In particular, it seems effective for small tumours (<3 cm), periductal tumours, and tumours abutting major hepatic vessels, where conventional local ablative treatments for such difficult tumour locations could be risky and less effective.
 
References
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