Functional profiling and strategic antimicrobial manipulation of a universal nutrition-sensing network to regulate microbial virulence, antibiotic tolerance, and stress protection
EWC Chan1, MTK Au2, RCY Chan2
1 Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University
2 Department of Microbiology, The Chinese University of Hong Kong
1. Nutrient limitation can trigger time-dependent onset of antibiotic tolerance phenotypes.
2. Starvation for 24 hours produced a large population of antibiotic persisters that reverted slowly to a drug-sensitive mode.
3. Inactivation of specific tolerance mechanisms has the potential to reduce the ability of an organism to develop antibiotic resistance.
4. Supplementation of small molecules such as amino acids is highly effective in abolishing antibiotic tolerance phenotypes.