Functional analysis of apoptosis-inducing factor in the human fungal pathogen Candida albicans
WK Tsang1, WP Fong2
1 Technological and Higher Education Institute of Hong Kong
2 Biochemistry Programme, School of Life Sciences, The Chinese University of Hong Kong
1. We have cloned and expressed the three putative Candida albicans apoptosis-inducing factor (AIF) sequences (orf19.1438, orf19.2175, orf19.2671) in Escherichia coli BL21. The proteins are designated as CaAifp-orf19.1438, CaAifp- orf19.2175, CaAifp-orf19.2671, respectively.
2. The three CaAifp have been purified from E coli BL21 to homogeneity.
3. Among the three CaAifp, only CaAifp-orf19.2175 and CaAifp-orf19.2671 possess NADH oxidase activity. The former also exhibits DNase activity.
4. CaAifp-orf19.2175 can functionally complement S cerevisiae Δaif1 mutant and sub-cellular translocation of this protein is evident upon apoptotic challenge.
5. CaAifp-orf19.2175 is a bona fide C albicans AIF.
2. The three CaAifp have been purified from E coli BL21 to homogeneity.
3. Among the three CaAifp, only CaAifp-orf19.2175 and CaAifp-orf19.2671 possess NADH oxidase activity. The former also exhibits DNase activity.
4. CaAifp-orf19.2175 can functionally complement S cerevisiae Δaif1 mutant and sub-cellular translocation of this protein is evident upon apoptotic challenge.
5. CaAifp-orf19.2175 is a bona fide C albicans AIF.