Expression analysis of putative small regulatory RNAs in Mycobacterium tuberculosis: effects of growth phase and oxidative stress
MTK Au, EWC Chan, RCY Chan
Department of Microbiology, The Chinese University of Hong Kong
Key Message
Results from northern blotting and RT-PCR can reveal which putative genes are authentic small RNA-coding elements and provide preliminary data on the induction characteristics of these important genetic regulators, thereby extending the number of small RNAs in MTB that are experimentally detectable at a transcriptional level, and fostering future research to delineate their regulatory role in this pathogen.