Hong Kong Med J 2013;19(Suppl 5):S12-4
Genome analysis of Mycobacterium tuberculosis Beijing family strains
SKW Tsui, RCY Chan, KM Kam, HS Kwan, KS Leung, KP Fung, MMY Waye, PTW Law, SK Lou, KK Leung
School of Biomedical Sciences, The Chinese University of Hong Kong
1. The genomes of the three 'totally drug-resistant' (TDR) Beijing/W strains of Mycobacterium tuberculosis (MTB) were sequenced and compared with publicly available genome sequences of KwaZulu-Natal MTB.
2. The sequences of the KwaZulu- Natal MTB strains shared a close ancestral relationship with MTB F11, whereas the three Beijing/W isolates formed a separate cluster.
3. The numbers of deletions, truncations, and frame-shift mutations were significantly greater in TDR Beijing/W strains.
4. Some DNA repair genes were defective in TDR Beijing/W strains.
5. Many genes involved in optimal mycobacterial growth were mutated in TDR Beijing/W strains.