Hong Kong Med J 2011;17(Suppl 6):S31-5
Pathogenesis of SARS coronavirus infection using human lung epithelial cells: an in vitro model
MCW Chan, RWY Chan, GSW Tsao, JSM Peiris
Department of Microbiology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong
1. In vitro models of well-differentiated bronchial epithelial cells and alveolar type II and type I–like pneumocytes were set up.
2. The SARS-CoV could replicate in well-differentiated bronchial epithelial cells and alveolar type II pneumocytes only.
3. In well-differentiated bronchial epithelial cells, type I interferons (interferon-beta) and cytokine and chemokines (eg RANTES, IP-10, IL-6, IL-8, MCP-1, MIP-1_, MIP-2_) were markedly induced after infection with SARS-CoV, compared to human coronavirus HCoV229E and influenza A (H1N1) virus.
2. The SARS-CoV could replicate in well-differentiated bronchial epithelial cells and alveolar type II pneumocytes only.
3. In well-differentiated bronchial epithelial cells, type I interferons (interferon-beta) and cytokine and chemokines (eg RANTES, IP-10, IL-6, IL-8, MCP-1, MIP-1_, MIP-2_) were markedly induced after infection with SARS-CoV, compared to human coronavirus HCoV229E and influenza A (H1N1) virus.