Hong Kong Med J 2011;17(Suppl 2):S32-4
Targeting glutamate synthase for tuberculosis drug development
YW Cheung, JA Tanner
Department of Biochemistry, The University of Hong Kong, Hong Kong
1. The Mycobacterium tuberculosis glutamate synthase consisting of alpha and beta subunits can be purified in a heterologous Escherichia coli expression system. 2. The beta subunit can be expressed in the soluble protein fraction under low temperature conditions.
3. The alpha subunit enters inclusion bodies in E coli but can be refolded and subsequently purified.