Hong Kong Med J 2009;15(Suppl 6):S39-43
Identification of human cell line model of persistent SARS coronavirus infection and studies of the response to cytokines and chemokines
KF To, PKS Chan
Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong
1. The human intestinal cell line LOVO, human lung epithelial cell lines NCI-H1650 and NCI-H1563, and normal nasopharyngeal epithelial cell lines NP69 and NP460 were permissive to SARS coronavirus (SARS-CoV) infection.
2. These human cell lines could be useful for future in vitro investigations of SARS.
3. The cytokine profiles induced following SARS-CoV infection varied among different cell lines, suggesting that the cellular response to SARS-CoV infection was tissue specific and may also correlate with the degree of permissiveness.
4. The results provide important insights into possible pathogenic mechanisms and potential novel therapeutic targets such as CXCL10/IP-10 in SARS.
2. These human cell lines could be useful for future in vitro investigations of SARS.
3. The cytokine profiles induced following SARS-CoV infection varied among different cell lines, suggesting that the cellular response to SARS-CoV infection was tissue specific and may also correlate with the degree of permissiveness.
4. The results provide important insights into possible pathogenic mechanisms and potential novel therapeutic targets such as CXCL10/IP-10 in SARS.