Hong Kong Med J 2008;14(Suppl 4):S4-7
Expression and functional characterisation of the putative SARS coronavirus non-structural proteins X1-X5
1. We produced mammalian expression vectors encoding the SARS coronavirus (SARS-CoV) accessory proteins with or without the fluorescence protein tag and cell lines with stable expression of these proteins.
2. The cellular localisation and function of the SARS-CoV accessory proteins was determined.
3. SARS 6 and SARS 8b proteins are localised to the endoplasmic reticulum and nucleus/cytoplasm, respectively, and both proteins stimulate host cell DNA synthesis.