Hong Kong Med J 2017;23:Epub 10 Nov 2017
Moderate iodine deficiency among pregnant women in Hong Kong: revisit the problem after two decades
WH Tam, MD, FRCOG1; Ruth SM Chan, PhD2; Michael HM Chan, FRCPA, FHKCPath3; LY Yuen, BSc1; Liz Li, MSc2; Mandy MM Sea, PhD2; Jean Woo, MD, FRCP2
1 Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong
2 Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, Hong Kong
3 Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, Hong Kong
Corresponding author: WH Tam (email@example.com)
Introduction: A survey conducted during 2005 to 2007 by the Centre for Food Safety in Hong Kong suggested that only 5% of the local population had a sufficient dietary intake of iodine. The study, however, was limited as biochemical data (ie urinary iodine concentration) were lacking. Pregnant women are vulnerable to iodine deficiency because of their increased requirement. Recent studies have shown that iodine deficiency in early pregnancy is associated with poorer cognitive development in early childhood. This study reports the iodine status of women during early gestation at an obstetric unit in Hong Kong.
Methods: Healthy pregnant women with no history of hyperemesis gravidarum were enrolled into a study when they first made a booking in an antenatal clinic of a public hospital to investigate their iodine status during early pregnancy. All subjects were asked to collect their morning urine for measurement of iodine and creatinine levels. Daily dietary intake of iodine was assessed in a subgroup of participants by structured interview using a standard food frequency questionnaire.
Results: A total of 600 pregnant women were enrolled at a median of 7.0 weeks of gestation. The median urinary iodine concentration and urinary iodine-to-creatinine ratio were 100 µg/L and 98 µg/g, respectively; 429 (71.5%) participants had iodine insufficiency according to the World Health Organization classification. Daily dietary intake of iodine was assessed in 146 participants. The median daily intake of iodine was 69.5 µg and 122 (83.6%) participants had an intake below the 250 µg recommended during pregnancy by the World Health Organization.
Conclusions: Local pregnant women continue to have an inadequate dietary intake of iodine and remain iodine-deficient.
New knowledge added by this study
- This study confirmed the previous observation that dietary intake of iodine is inadequate in our local population, in particular in women during pregnancy.
- The majority of the pregnant women were taking multivitamin supplements without iodine, suggesting inadequate knowledge about the importance of iodine.
- There is an urgent need to educate the general public, in particular women of childbearing age, about the importance of an adequate dietary intake of iodine and the option of iodine-containing multivitamin supplements before contemplating pregnancy.
- We can consider a policy to monitor the iodine status of the population, especially in vulnerable groups including pregnant and lactating mothers, and their children.
- World Health Organization recommends salt iodisation in countries and areas that are iodine-deficient. As less than 15% of table salt available in Hong Kong is iodised, it may be appropriate to consider this policy.
The World Health Organization (WHO) considers iodine deficiency the single most important preventable cause of brain damage worldwide. Since 1993, WHO has recommended universal salt iodisation to eliminate iodine deficiency disorder.1 There is a common belief that the Hong Kong population resident in coastal regions should have sufficient iodine intake. Twenty years ago, however, 50% of children and adults were found iodine-deficient according to the WHO standards.2 At the same time, studies also reported that one third of local pregnant women were iodine-deficient based on their urinary iodine concentration (UIC), from the first through third trimester.3 4 Pregnant and lactating women are among the most vulnerable groups in the population as iodine plays an important role in early neuronal migration and maturation in the developing fetus and infants.
A local expert panel group was established in 2003 to encourage the monitoring of iodine status and rectify the problem.5 The panel concluded that dietary iodine intake of our population was borderline sufficient, and was also inadequate to meet any extra requirement at the time of thyroid stress, ie pregnancy, neonatal period, and in the first few years of life.5 In a local population survey conducted by the Centre for Food Safety during 2005 to 2007, the median iodine dietary intake was as low as 44 µg/day among 5000 adults, while 60% had an intake of <50 µg/day (the threshold for normal thyroid functioning).6 Only 5% had an iodine intake within the safe range. This report, however, also admitted a lack of clinical and biochemical data (ie UIC) that precluded a conclusion about iodine deficiency. Since this report in 2011, all mothers are enquired about their dietary intake of iodine and provided with iodine-containing multivitamin supplements when they book in at a Maternity and Child Health Centre in Hong Kong.
Nevertheless, the problem remains unresolved as two recent cohort studies in the UK and Australia consistently reported that low maternal first-trimester UIC was associated with poorer childhood cognitive development at the age of 8 to 9 years.7 8 The investigators of the Avon Longitudinal Study of Parents and Children in the UK also reported a dose-effect association; child neurocognitive scores were lower when maternal urinary iodine-to-creatinine ratio (UICr) was <50 µg/g.7
As no salt iodisation programme has been implemented since the first study was carried out, and with the changes in social circumstances and dietary trends over the last two decades, it was deemed necessary and important to reassess the iodine status of pregnant and lactating women in Hong Kong to guide future policy. The objective of this paper was to report the iodine status of women during early gestation at an obstetric unit in Hong Kong.
Between July 2014 and November 2015, healthy pregnant women were enrolled at the antenatal clinic of Prince of Wales Hospital into a study of gestational age-specific thyroid function test reference intervals. Women without a history of thyroid dysfunction, hyperemesis gravidarum, autoimmune disease, or any other major medical conditions were eligible. Consecutive cases were recruited at their first attendance for antenatal booking. We estimated that 250 blood samples were required for each block of gestational age range (<6, 6-10, >10-14, >14-18, >18-24, >24-32, >32-38, and >38 weeks) to generate a nomogram; 600 subjects were required to provide 2000 samples, each had an average of four blood samples across the gestation, with an estimated dropout rate of 15%. All subjects underwent early ultrasound scan for dating with gestational age adjusted according to the ultrasound date if it differed to that calculated from the last menstrual period. The study was approved by the Chinese University of Hong Kong Clinical Research Ethics Committee (CRE-2013.500), and written informed consent was obtained from all women.
As part of the study design, UIC was measured at the time of recruitment to determine the participant’s iodine status. Women were asked to collect a morning urine sample into an acid-washed trace element urine bottle within 1 week of recruitment for the assay. In this study, UIC was measured by an inductively coupled plasma mass spectrometer (ICP-MS 7700; Agilent Technologies, US). The intra- and inter-assay coefficients of variation for UIC were 3.3% and 7.4% at 11.9 µg/L, and 2.7% and 4.9 % at 49.6 µg/L, respectively. Urinary creatinine concentration was also measured by IDMS-traceable Jaffe kinetic reaction (cobas 8000; Roche Diagnostics Inc, US) to calculate the UICr.
All participants were interviewed by a research nurse who asked about the duration, frequency, and brand of any multivitamin supplements taken to quantify daily iodine supplementation. Due to the limited funding, dietary intake of iodine-containing foods was only assessed in a conveniently sampled subgroup of the population by another research assistant using a food frequency questionnaire (FFQ; Appendix). Education level and occupation were recorded for this subgroup. The FFQ was based on the literature and previously validated FFQs that have been used in the local population.6 9 10 Eleven main food groups that contribute to iodine intake in the local population were incorporated into the FFQ (Table 1). Portions were reported based on standard reference sizes, eg cups, grams, centilitres. To facilitate understanding and recall, food photo albums and eating utensils of standard portions were presented when completing the FFQ. Use of iodised salt was also documented. Daily dietary intake of iodine was calculated using Food Processor Nutrition Analysis and Fitness software, version 8.0 (ESHA Research, Salem, US). The iodine content of local foods was also programmed into the software, extracted from food composition tables from Hong Kong SAR and Mainland China.11 We used the WHO definition to categorise insufficient, adequate, above requirements, and excessive by UIC of <150, 150-249, 250-499, and ≥500 µg/L, respectively12; UICr of <150 µg/g was also used to define insufficiency according to Bath et al.7 An average daily iodine intake of 250 µg/day was considered adequate for pregnancy.1 Data are expressed as mean ± standard deviation (SD), median and interquartile range (IQR), or counts with proportion. Between-group differences were compared using the Student’s t test and χ2/Fisher’s exact tests, as appropriate. Multivariate logistic regression analyses were used to obtain adjusted odds ratios with 95% confidence intervals, with the forced entry of covariates, namely maternal age, parity, education level, occupation, body mass index, and gestational age at recruitment, to assess factors associated with low UIC (<150 µg/L), low UICr (<150 µg/g) and iodine-containing supplementation. Multivariate linear regression analyses were used to assess the association with UIC and UICr. Statistical analysis was performed using SPSS (Windows version 20.0; IBM Corp, Armonk [NY], US). A P value of <0.05 was used to indicate significance for two-tailed statistical test results. The gestational age-specific thyroid function test reference intervals are not included in this manuscript but will be published elsewhere.
A total of 600 healthy pregnant women were enrolled during the study period at a median gestational age of 7.0 (IQR, 5.9-8.6) weeks. The mean (± SD) age and body mass index at recruitment were 31.3 ± 3.9 years and 21.9 ± 3.1 kg/m2, respectively. Of the subjects, 382 (63.7%) women were nulliparous and two (0.3%) were twin pregnancies. The median (IQR) UIC and UICr were 100 (58-165) µg/L and 98 (67-150) µg/g, respectively (Table 2). According to the WHO definition, 429 (71.5%) participants were regarded as iodine deficient (Fig a). Moreover, 450 (75.0%) participants had UICr of <150 µg/g (Fig b).7
Table 2. Characteristics of all 600 participants and the subgroup of participants who completed the FFQ
Figure. Subjects classified according to the WHO definition on the basis of (a) urinary iodine concentration, and (b) urinary iodine-to-creatinine ratio in the study population
Daily dietary intake of iodine was assessed in 146 participants. They were slightly younger in age and had a higher rate of nulliparity; their UIC and UICr were no different to all participants (Table 2). The median (IQR) daily dietary intake of iodine was 69.5 (47.3-152.4) µg. The greatest source of iodine among the studied sample was from seaweeds that were consumed by 76% of women (Table 1). The next most common source of iodine was non-alcoholic beverages such as soy milk, soup, soft drinks, and water (21.5% of the daily iodine intake). Of all the 146 women surveyed, only four (2.7%) regularly used iodised salt in their diet. Several food sources were commonly eaten by all women including fish, cereal and grain products, vegetables and legumes, and condiments. Nonetheless these combined food sources only contributed to 15.5% of daily dietary iodine intake. There were 44 (30.1%), 92 (63.0%) and 122 (83.6%) participants who had a daily iodine intake below 50, 100, and 250 µg respectively.
Nutritional supplementation was reported by 414 (69.0%) of all participants at the time of enrolment but only 163 (39.4%) of these supplements contained iodine; the daily iodine supplement was between 140 and 290 µg if taken according to the prescription. The subgroup of participants who had taken iodine-containing supplements for 2 weeks or more had an adequate iodine status both by their median UIC and UICr (Table 3). In 122 subjects who completed FFQ and had urinary iodine level measured within 1 week of each other, total daily iodine intake (iodine supplementation included) had a weak correlation with UICr (r=0.20, P=0.03). In multivariate regression analyses, no covariates except gestational age at recruitment were associated with low UIC or UICr, or iodine supplementation; women recruited at a later gestational age had significantly higher UIC and were more likely to have started taking an iodine-containing supplement (Table 4).
Table 3. Median UIC and UICr among participants who did not take iodine supplements and those who took it for more than 1 or 2 weeks
The findings from this study suggest that local pregnant women might still be iodine-deficient when the WHO definition is applied. In the subgroup of participants who completed the FFQ, more than 80% did not have an adequate daily iodine intake as recommended by the WHO and other authorities.13 14 This is in keeping with the survey conducted by the Centre for Food Safety 10 years ago. A recent local study of 95 lactating mothers showed that the mean daily dietary intake of iodine was 62.6 µg with only 2% having a sufficient iodine intake.15 The result also showed that only 48% of breast milk samples from 39 women with an infant younger than 6 months had an adequate level of iodine (85 µg per day) as recommended by the Chinese Dietary Reference Intake.15 The revised guideline from the Department of Health in 2016 suggested that mothers consider taking iodine-containing prenatal supplements during pregnancy and breastfeeding in view of the difficulties in obtaining sufficient iodine from food alone.16 The policy appears to be in agreement with our findings that mothers taking iodine-containing supplements were iodine sufficient according to their median UIC. Nonetheless universal iodine supplementation remains controversial. The American Thyroid Association, Endocrine Society, and New Zealand Ministry of Health recommend that women who are planning a pregnancy or who are currently pregnant or lactating should receive 150 µg per day of iodine supplementation in the form of potassium iodide-containing supplements.17 18 On the contrary, Cochrane systematic review did not support routine iodine supplementation in women before, during or after pregnancy, while WHO does not recommend iodine supplementation in regions where the median UIC indicates iodine sufficiency, or where a salt iodisation programme is in place.1 19 All eight iodine-containing supplements that were reportedly taken by participants in this study also contained iron, and frequently resulted in varying degrees of constipation. Most women did not start taking supplements prior to their first antenatal visit. Even in those who did, 60% of the supplements contained no iodine, similar to that reported in the US.20 21 Our results also show that mothers were more likely to have commenced iodine supplementation and have an iodine adequate status at a later gestation; this suggests mothers might acquire the knowledge as pregnancy progresses.
Although the UIC is designated for assessment of a population, not individual iodine status, our results suggest that three quarters of the participants may have been iodine insufficient during early pregnancy according to their UIC and UICr. Similarly, iodine deficiency in pregnancy has re-emerged in several developed countries.22 Subjects enrolled into the Avon study in the UK were mildly-to-moderately iodine deficient with a median UIC of 91 µg/L.7 A more recent study among schoolgirls in the UK also reported a similarly low UIC suggesting that mild-to-moderate iodine deficiency remains a problem.23 Such deficiency was probably caused by a poor availability of iodised salt, few UK recommendations for increased iodine intake in pregnancy, and insufficient use of iodine-containing prenatal supplements. The International Council for Control of Iodine Deficiency Disorders global network now places the UK on the list of mildly deficient nations.24 The Department of Health in the UK has also added iodine to its National Diet and Nutrition Survey that checks the nutrient intake of adults and children in the UK.25 In Australia, mandatory iodine fortification of salt used in bread was introduced in 2009 in order to tackle mild iodine deficiency in the population; iodine status of women has improved since then but only those taking iodine-containing supplements have UIC indicative of sufficiency.26 According to data from the National Health and Nutrition Examination Survey 2005-2010, more than 55% of pregnant women had UIC that suggested inadequate iodine intake.27 Mainland China has led the way in sustaining the elimination of iodine deficiency through iodised salt since the early 1990s.28 Nonetheless dietary iodine intake remains insufficient among pregnant women in Shanghai, Zhejiang, and other coastal cities where iodine is considered sufficient for the general population.29 30 A national survey from the Mainland reported that the median UIC levels of pregnant and lactating women were 123-224 µg/L and 109-224 µg/L, respectively; median UIC was higher among those in inland cities, because of a higher iodine level in the salt and greater household coverage.31 In contrast, the progress in tackling iodine insufficiency in Hong Kong has been far from satisfactory.
Due to the limitations in study design, this study can only provide a snapshot overview of the iodine status of local pregnant women during early pregnancy from a single obstetric unit. Given that the main objective of the study was to ensure our study group had sufficient iodine intake in order to establish a thyroid function test reference range for the local population, our results cannot draw any conclusions about iodine status during the second and third trimesters. Moreover, dietary iodine intake could only be assessed in a subgroup of the population due to limited funding. The WHO has considered iodine deficiency the single most important preventable cause of brain damage worldwide. Recent study also highlighted the impact of iodine deficiency in the first 1000 days of life especially among breast-fed infants.32 As the Hong Kong SAR Government is actively promoting breastfeeding, it is also important to ensure adequate iodine in lactating mothers and breast-fed infants. It is time to systematically revisit the iodine status of our local women at pre-conception, and during pregnancy and lactation.
Results of our study are in line with those from the survey by the Centre for Food Safety, suggesting that our local pregnant women are borderline iodine-deficient and have an inadequate dietary iodine intake during early pregnancy. There is a need to educate the public and to advise women of childbearing age to maintain sufficient dietary iodine before contemplating pregnancy. A policy of salt iodisation and regular monitoring of iodine status with UIC in our population should be considered.
This study was supported by the Hospital Authority of Hong Kong on a project to derive a gestational age-specific thyroid function reference interval for the local pregnant population. Ms Sharon Lai-kwai Chan, research nurse, recruited all subjects, and collected and organised the clinical data. Ms Macy Kwan conducted face-to-face interviews for the dietary questionnaire for the subgroup of subjects.
All authors have disclosed no conflicts of interest.
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