Differential gene expression profile of CD4+/CD8+ T cells in patients with hepatitis C virus and/or human immunodeficiency virus infection
L Yi, J Zhao, J Lu, Y Chen, D Huang, YF Li, L Chen, ZR Yang, HF Kung, ML He
Stanley Ho Centre for Emerging Infectious Diseases, Faculty of Medicine, The Chinese University of Hong Kong
Key Messages
1. Coinfection of a host organism with hepatitis C virus (HCV) and human immunodeficiency virus (HIV) alters the course of infection of each virus.
2. Treatment-naive HIV/HCV mono-/co-infected patients with CD4+ T cell count >300/µL were recruited, and the gene expression profile of their CD8+ and CD4+ T cells was investigated by microarray assay, bioinformatics analysis, and quantitative real time PCR validation.
3. Genes involved in cell proliferation, activation, differentiation, and regulation and cytokine responses were significantly altered in CD8+ T cells. In CD4+ T cells, innate immune response, cell cycle regulation, GPCR signalling pathway, transcriptional regulation, and metabolic pathways were significantly affected by HIV/HCV co-infection, compared with HCV mono-infection.
4. These findings offer new insight into disease progression in HIV/HCV co-infection, and may help to identify new markers for its management.